ACS BITS
Suspension cloning is particularly for the following cells [ a ]a. Hematopoietic cells b. Neoplastic c. Bone Marrow d. Embryogenic
Cell isolation by irradiation is achieved by ___ rays [ a ]
X rays b. ¡ rays c. Cosmic rays d. U. V rays
Epidermal cells will selectively grown is ____ media [ b ]
a. Serum free media b. Feeder layers media
c. Serum defined media d. Completed layers media
Simple system for scaling up of monolayer culture is [ a ]
a. Nucleon cell factory b. Munclone cell factory
c. Punclone cell factory d. Ouclone cell factory
Micro encapsulation of animal cells is achieved by the following compound [ a ]
a. Sodium alginate b. Sodium nitrate c. Sodium phosphate d. sodium acetate
Isolation of pure cell line is done by [ b ]
a. Selection b. Cloning c. Micromanipulation d. Preservation
Most common medium used for cloning is [ c ]
a. Agra medium b. Bam’s F12 medium c. Han’s F12 medium
d. Beef extract medium
Hormone which is most commonly used in cloning of animal cell culture is [ c ]
a. Pituitary hormone b. Parath hormone c. Insulin hormone
d. Glycogen hormone
------------------ is synthetic analog which improves the plating efficiency of cell types [ a ]
a. Dexamethasone b. Hexamethasone
c. Phosphomethasone d. Chloromethasone
Supplemented medium for cloning contains ------------------ keto acids [ c ]
a. Gluta Mate – Glutamate b. Alamine – Valanine
c. Pyruvate - a Keto glutarate d. Succinate – Malate
---------------- will improves the plate efficiency of human fibroblasts in low serum concentrations human fibroblasts in low serum concentrations [ d ]
a. Poly glycine b. Poly cystine c. Poly leucine d. Poly lysine
---------------------- cells are readily clone in suspension cloning technique [ b ]
a. Stem cells b. Hematopoietic stem cells
c. Embryonic stem cells d. Bone marrow cells
Transformed cells have ------------ serum dependency than their normal counter parts. [ b ]
a. Higher b. Lower c. Moderate d. Very high
Tumor cell produce ---------------- types of growth factors [ c ]
a. Embryonic b. Hemolytic c. Hemopoetic d. Erythropoietic
------------------ are most commonly used for roller culture in large numbers. [ a ]
a. Roller Drum apparatus b. Roller Plate apparatus
c. Roller Dish apparatus d. Roller Chamber apparatus
Surface area for Monolayer growth has been increase by [ b ]
a. Multi array Vials b. Multi array Disks c. Multi array Brakers
d. Multi array Petri plates
---------------- cells cannot be grown in liquid suspension medium [ a ]
a. Anchorage – dependent cells b. Serum media – dependent cells
c. Protein free – dependent cells d. Addhission – dependent cells
In scale up suspension culture sparging is done by [ d ]
a. O2 b. H2 c. N2 d. Co2
------------------ is most commonly used to show DNA content of tumor cells [ b ]
a. Spectrophotometer b. Flow cytometer
c. Calorimeter d. Nuclear magnetic spectrophotometer
Transfection can be estimated by ------------ [ a ]
a. Amount of DNA b. Amount of RNA
c. Amount of Proteins d. Amount of Carbohydrates
____stem cells used to study differentiation of animal cells [ a ]
Embriyonal b. Zygotic c. Poiener d. Placental
Human adult stem cells are isolated by ____ [ b ]
Mechanical sorting with ACAS b. Magnetic sorting with FACS
c. Chemical sorting with CMAS d. Electrical sorting with AGCS
Multi potent stem cells are most commonly seen in ____ [ c ]
10 to 15 years b. 15 to 20 years c. 20 to 25 years d. Above 50 years
Cultures derived from embryoid bodies are isolated from [ c ]
PG cultures b. CG cultures c. EG cultures d. AG cultures
Human tetra tocarcinomas cells are propagated in 118 medium which consists of [ d ]
EGF – 1 b. EGF – II c. FGF – I d. FGF – II
Fusion of somatic cells is achieved by [ b ]
a. Mercapeto ethanol b. Poly ethylene glycol
c. Poly ethylene d. Poly methylene
Viable hybrids of somatic cell fusion is grown in [ d ]
a. Agar medium b. Beef extract medium c. MAT medium
d. HAT medium
Protocol for somatic cell fusion of animal cell is given by [ d ]
a. Ian Healy b. Von – Leawonhock c. Ivor Belfast
d. Ivor Hickey
Myeloid cell lines are developed from [ d ]
a. Murine Carcinomas b. Murine Sarcomas
c. Murine Lymphomas d. Murine Leukemias
Culture medium of T-cell lines requires -------------- growth factor [ b ]
a. Inter forons – I b. Inter leukin – II
c. Inter feron – II d. Inter leukin – I
Human lymphoblastoid cell lines were derived for the first time from ------------- [ ]
a. Lymphocytes b. Monocytes c. Leucocytes d. Blastocytes
Erythroleukemia cell lines are developed from the ------------------ of erythroblastosis infected mice. [ d ]
a. Minced Brain b. Minced Liver c. Minced Kidney d. Minced Spleen
Treating by animal hematopoietic stem cells is done by [ d ]
a. Acetalddhyde b. Farmal dehyde c. Oxalic acid d. Isobutyric acid
Treated hematopoetic stem cells shows the following features. [ a ]
a. Nuclear condensation b. Nuclear Polymerization
c. Nuclear Compartmentalization d. Nuclear differentiation
Evidence for differentiation of haematopoietic stem cells is demonstrated by [ d ]
a. Staining with sapharine b. Staining with Lushmann’s stain
c. Staining with Phenapthalene stain d. Staining with Benzedine
Isolation of multipotent stem cells are done from [ b ]
a. Serum b. Bone marrow c. Kidney d. Blood
Embryonal stem cell line is derived from [ a ]
a. Preimplanted embryo b. Postimplanted embryo
c. Implanted embryo d. Non implated embryo
Examples of embryonal stem cell lines are [ d ]
a. ES – A3, B1, C1 b. ES – B3, A1 & C1 c. ES-C3, A1 & D1
d. ES – D3, J1 & R1
Examples of embryonal carcinoma cell lines are [ d ]
a. A9 & B19 b. D9 & F19 c. E9 & G19 d. F9 & P19
Differentiation of stem cells is promoted by [ a ]
a. Retinoids & Cytokines b. Carotinoids & Lymphokines
c. Xanthophylls & Leucopenes d. Steoids & Lymphokines
Organotypic typic culture is derived from _____ [ c ]
a. Homogenous primary culture b. Homogenous secondary culture
c. Heterogeneous primary culture d. Heterogeneous secondary culture
Histotypic culture is defined as _____ [ a ]
a. Cell propagation in 3 dimensional matrix
b. Cell propagation in 1 dimensional matrix
c. Cell propagation in 2 dimensional matrix
d. Cell propagation in multi dimensional matrix
Significance of organo typic culture is ___ [ b ]
a. It retains culture properties b. It retains original structural relations of cells
c. It retains permeability of cells d. It retains primary cell relations
Medium used for organ culture is ___ [ a ]
a. M – 199 b. M – 200 c. M – 195 d. M – 198
In sphemoids type of histo typic culture medium contains ____ agar [ c ]
a. Solid agar b. Semi solid agar c. Noble agar d. Trpsin free agar
Increase O2 concentration in Organotypic culture facilitates -------------- [ d ]
a. Hyperbaric exchange b. Ion exchange fromcells
c. Gaseous exchange d. Nutrient metabolic exchange
Organ culture is essential for studying the behaviour of ------------------ [ c ]
a. Integrated cells b. Nonintegrated cells c. Integrated tissues
d. Nonintegrated tissues
How many types of histotypic cultures are there [ c ]
a. 4 b. 7 c. 8 d. 9
Malignant cells penetration through cellulose sponge is facilitated by [ b ]
a. Trypsin coating b. Collagen coating c. Gel foam d. Gel coating
------------------ is used as matrix Morphogenesis of primitive epithetical cells [ a ]
a. Collagen gel b. Gel foam c. Gel coating d. Collagenase
----------------- is used as matrix for formation of capillaries from endothelial cells. [ c ]
a. Collagen gel b. Gel foam c. Matrigel d. Calomel gel
Chief constituent in the composition of Matrigel is -------------- [ a ]
a. Fibronection b. Catrilage c. Cellulose d. Hemicellulose
------------------ are most commonly used in the modeling of vascular tumour growth. [ c ]
a. Gel sponge b. Hollow fibers c. Spheroids d. Collagen Sponge
Example for Rotating Chamber system is [ a ]
a. Miniperm Bioreactor b. Maxiperm Bioreactor c. Megaperm Bioreactor
d. Minuteperm Bioreactor
Alginate is most commonly seen in [ d ]
a. Brown algae b. Red algae c. Brown Seaweed
d. Red seaweed
Carbohydrate composition of Alginate is -------------------- [ c ]
a. Glucose and Galactose b. Glucose amine & galactosamine
c. Glucouronic acid & Mannuronic acid d.Glucose amine & Mannose amine
Alginate encapsulation – protocol is first given by [ a ]
a. TA Read & R. Bjerkvig b. T.A soon & Shiong
c. Offerlei & Miura d. Halleux & Schneider
Necrosis of a cell is triggered by [ a ]
a. Gross damage b. Degeneration of cell c. Lysis of cell
d. Regeneration of cell
Apoptosis is initiated by [ a ]
a. Result of inappropriate set of signals b. Result of Physical trauma
c. Result of appropriate set of signals d. Result of appropriate set of aging
Static measure of growth of cell is [ b ]
a. Size of cell b. Cell number c. Dividing of cell
d. Increase in Nuclear material
3 dimensional cell cultures have been widely used in ____ [ b ]
a. Biochemical research b. Biomedical research
c. Biotechnical research d. Biological research
Major advantage of 3 dimensional cell cultures is ____ [ b ]
a. Well defined cytology b. Well defined geometry
c. Well defined cell structure d. Well defined cellular organelles
Identity to component of tissue engineering from the following [ a ]
a. Material science b. Natural science c. Biological science d. Life science
Implanted bladders developed by tissue engineering achieved normal performance for up to
___ months [ c ]
a. 6 b. 8 c. 11 d. 12
Support material used for tissue engineering under synthetic category is [ b ]
a. Biopolymers b. Resorbable polymers c. Synthetic Polymers
d. Organic polymers
Investigations on micro environmental and epigenetic mechanisms are involved in the regulation of ---------------------- [ a ]
a. Cell proliferation b. Cell reproduction c. Cell separation
d. Cell adhission
Antibiotics used in tumour spheroods culture in spinner flask. [ c ]
a. Geneta mycin b. Nova mycin c. Strepto mycin d. Pleuromycin
Medium used in cultivation of liquid overlay tumour spheroid cultures. [ b ]
a. RPPM medium b. RPMI medium c. PRMM medium d. MPRM medium
------------------ is used for fixation of Monolayers in liquid overlay culture [ a ]
a. Acetone b. Phenol c. Acetic acid d. Chloroform
Freezing is achieved by ----------------- in liquid overlay culture. [ c ]
a. Dry freezing b. Liquid freezing c. Liquid N2 freezing
d. Deep freezing
------------------- medium used in culture of endothelial cell is [ b ]
a. Mam’s F12 & Agar medium b. Ham’s F12 & Iscove’s medium
c. Bam’s F12 & Procoves d. Ram’s F12 & Brocoves medium
-------------- solution is used to fill inside the umbilical cord on cultivation of human umbilical vein endothelial cells [ b ]
a. Peps solution b. Hepes solution c. Bepes solution d. Mepes solution
Systematic monitoring of migration of tumour cells is done by using [ b ]
a. Inverted microscope b. Phase contrast microscope
c. Electron microscope d. Binocular microscope
Enzyme used in cultivation of oral mucosal cells [ c ]
Nerve guidance was achieved by----------------- in tissue engineered peripheral nerve implants. [ a ]
a. Silicone b. Alumina c. Cobalt d. Nickel
Natural polymers used as supporting materials in tissue engineering are [ d ]
a. Porphyries b. Lipids c. Nucleic acids d. Proteins
Traditional materials used as supporting materials in tissue engg. Are [ a ]
a. Ceramics b. Polymers c. Plaster of Paries d. Polysaccharides
Antibiotic used in human dermal fibroblast seeding culture is [ a ]
a. Penicillin b. Pleuromycin c. Acetomycin d. Zentamycin
Growth factors used in the slow release growth factor depot formation is ---------- [ c ]
a. Interleukine like growth factor b. Insulin like growth factor
c. Nerve growth factor d. Mesodermal growth factor
Medium used in urothelial cell seeding on collagen sponges culture is ----------------- [ a ]
a. RPMI medium b. GPMI medium c. MPMI medium d. PPMI medium
No comments:
Post a Comment